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    <title>ms</title>
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    <id>tag:blogs.perl.org,2009-11-03:/users/ms//1768</id>
    <updated>2013-02-01T00:32:57Z</updated>
    <subtitle>A blog about the Perl programming language</subtitle>
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<entry>
    <title>what to know about aligning</title>
    <link rel="alternate" type="text/html" href="http://blogs.perl.org/users/ms/2013/02/what-to-know-about-aligning.html" />
    <id>tag:blogs.perl.org,2013:/users/ms//1768.4245</id>

    <published>2013-02-01T00:17:37Z</published>
    <updated>2013-02-01T00:32:57Z</updated>

    <summary>Hi I am a biologist not a bioinformatician, I have two group of sequences (they are nucleotide and in fasta format), each group includes around 40,000 sequences ranging from 100 bp to 12 kb. I want to know how can...</summary>
    <author>
        <name>ms</name>
        
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        <![CDATA[<p>Hi<br />
I am a biologist not a bioinformatician,  I have two group of sequences (they are nucleotide and in fasta format), each group includes around 40,000 sequences ranging from 100 bp to 12 kb. I want to know how can I align the sequences from a group to the another and find the best pair for each fragment. Can I do it through Perl? if so how can I do that? is there any softwares that can I use? <br />
second Q<br />
how can I find secondary structures of the sequences in each groups? which program should I use?<br />
Thanks<br />
MS </p>]]>
        
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