March 2020 Archives

Comparing coronavirus SARS-CoV-2 spike protein sequences with BioPerl

Continuing from extraction of coronavirus spike protein sequences I decided to compare them and see if any mutations could be found. To do this I needed to align sequences to each other and get multiple sequence alignment. There are many tools that might be used and I have chosen MUSCLE as it is fast, easy to use and accurate enough. However,…

Extracting coronavirus SARS-CoV-2 spike protein sequences with BioPerl

As I started learning Perl I found out that there is a collection of Perl modules for bioinformatics tasks and it is called BioPerl. Intrigued I decided to try to use it for a simple task as it would help me in learning Perl. So the next question was: what task should I choose? Maybe download file with biological sequences, parse it and filter according some criteria? Thinking about this further I made a choice. I will work with sequences of infamous coronavirus which is more precisely named as /users/zubenel/2020/03/index.html

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